Composite

Part:BBa_K3583008:Design

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Escherichia coli PPK mutant E173K with vacuolar import peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 972
    Illegal BamHI site found at 353
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal AgeI site found at 2326
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon of the enzyme has to be moved behind the plant specific insert.


Source

Check the pages of each Biobrick used.

References